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- W2380472008 abstract "Objective:To investigate the experimental conditions of radionuclide iodine(131I) labeling epidermal growth factor receptor(EGFR) monoclonal antibody(McAb) 1H12,observe the cell-binding function and biological effects of 131I-1H12 to human lung cancer cells A549.Methods:1H12 was labeled with 131I by Iodogen method.The immunocompetence of 131I-1H12 was analyzed by cell-binding test.The cell apoptosis and cell cycles of A549 were analyzed by flow cytometry assay with various doses of 131I-1H12(148,74,37 kBq),free131 I(148 kBq),1H12(80 nmol·ml-1) and equivalent medium.Results:The labeling rate of 131I-1H12 was 50.14%,its specific activity,radioactive concentration and radiochemical purity were 4.63 MBq·μg-1,77.31 MBq·ml-1 and 96.92% respectively.The specific binding rate of 131I-1H12 to A549 cells was 61.12% ;The apoptosis rate and cycle blockage rate of A549 cells induced by 131I-1H12 were in a dose-effect relationship;The supreme apoptosis rate[(44.35 ±3.31) %]and G2 /M cell cycle blockage rate[(51.17 ±2.98) %]were found in the A549 cells treated with 148 kBq 131I-1H12.Conclusion:131I-1H12 can immunobind with A549 cells and regulate cell cycle and induce apoptosis of A549 cells to inhibit its proliferation;It might have some potential application prospect in biological targeted diagnosis and therapeutics." @default.
- W2380472008 created "2016-06-24" @default.
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- W2380472008 date "2010-01-01" @default.
- W2380472008 modified "2023-09-23" @default.
- W2380472008 title "Preparation of ~(131)I-1H12 and its biological effects on non-small cell lung cancer cell A549" @default.
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