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- W2380474756 abstract "Objective:To investigate the factors affecting in vitro transfection efficiency mediated by chitosan(CS) using the enhanced green fluorescent protein plasmid(pEGFP) as reporter gene.Methods:pEGFP/CS nanoparticles were prepared by the complex coacervation method.The effects of the molecular weight of CS,N/P ratio,particle size of the nanoparticles,pH of transfection medium and the presence of serum on transfection efficiency were investigated in HEK293 cells.Results:The transfection efficiency of pEGFP/CS nanoparticles was found to be highest when the molecular weight of chitosan was 5 KDa or the pH of transfection medium was no more than 6.8.Moreover,the transfection efficiency was independent on the particle size in the range of 230 to 1 290 nm and was enhanced by the increase of N/P ratio.Furthermore,the presence of serum had no impact on the CS-mediated transfection efficiency.Conclusion: The molecular weight of CS,N/P ratio and pH of transfection medium were the important factors which would affect transfection efficiencies mediated by CS.Low molecular weight and water-soluble CS was advantageous to improving transfection efficiency." @default.
- W2380474756 created "2016-06-24" @default.
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- W2380474756 date "2008-01-01" @default.
- W2380474756 modified "2023-09-24" @default.
- W2380474756 title "Chitosan nanoparticles as gene vector:factors affecting in vitro transfection efficiency" @default.
- W2380474756 hasPublicationYear "2008" @default.
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