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- W2380476965 abstract "Objective: In order to find an optimal method to cultivate embryonic neural stem cells in vitro,we compared the effect of different supplement N2 and B27 with different concentrations on the proliferation of neural stem cells.Methods: The embryonic neural stem cells were isolated from 13.5 d SD rat.The effects of different supplements N2 and B27 with different concentrations on the proliferation of neural stem cells were investigated and compared by the methods of CCK-8 counting,BrdU administration and measuring neural ball diameter.Results: The combination group of N2 and B27 had most obvious effect on the proliferation and formation of neuro spheres of neural stem cells;the effect of 2% B27 alone was better than that of 1% B27 group and N2 group.Conclusion: The combined supplement of 1% N2 and 1% B27 were more conductive to the proliferation and self-renewal of neural stem cells,it will provide experimental bases for the application of neural stem cells." @default.
- W2380476965 created "2016-06-24" @default.
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- W2380476965 date "2012-01-01" @default.
- W2380476965 modified "2023-09-23" @default.
- W2380476965 title "Effects of Supplements N2 and B27 with Different Concentrations on the Proliferation of Neural Stem Cells" @default.
- W2380476965 hasPublicationYear "2012" @default.
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