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- W2380478636 abstract "Objective To construct the cDNA library of adult Clonorchis sinensis and screen the fu nctional gene. Methods Construct the cDNA library of adult Clonorchis sinensis by SMART method and sequence a large quantity of EST.Compare the sequence of EST with that reported in GenBank for analysis of homology,then perform sequence splicing and analyze g ene integrity.Analyze the conserved domain of the screened gene by RPSBLAST prog ram in NCBI software and predict its functional domain and secondary structure b y PredictProtein program. Results The calcineurin B-like protein(CsCBLP) gene of adult Clonorchis sinensis wa s screened.The homologies of CsCBLP gene to the CaN of Rattus norvegicus,CaN B of Naegleria gruberi and binding protein of Caenorhabditis elegans we re 53%,40% and 51% respectively.The analysis of conserved domain as well as the prediction of functional domain and secondary structure proved that the screened CsCBLP was analogous to calcineurin B belonging to calcium binding protein fami ly. Conclusion CsCBLP gene was screened from the cDNA library of adult Clonorchis sinensis by bioinformatical method." @default.
- W2380478636 created "2016-06-24" @default.
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- W2380478636 date "2005-01-01" @default.
- W2380478636 modified "2023-09-23" @default.
- W2380478636 title "Screening of Calcineurin B-like Protein Gene,The Functional Gene of Adult Clo norchis sinensis by Bioinformatical Method" @default.
- W2380478636 hasPublicationYear "2005" @default.
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