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- W2380480069 abstract "Objective To establish the animal model of heterotopic spleen transplantation in rats with stent and cuff technique.Methods 30 operations of spleen transplantation were performed with 60 health mile Wistar rats. In the operation, the donor celiac artery and the recipient renal artery were anastomosed with a stent of 22G polyethylene tube, and the donor portal vein and the recipient renal vein were anastomosed with a cuff of 17G polyethylene tube. The survival status of the recipients and the morphometric change were observed and evaluated postoperatively. Results All of the 30 operations were successful. Most recipient rats survived well after operation except for 4 rats (1/4 die for thrombus, 2/4 for pancreatitis, and 1/4 for abdominal infection). The graft spleens became swollen on day 3 after grafting, gradually became hard and patchy and small, then white and necrotic on day 14, and finally were absorbed. Histological change of grafted spleens manifested as following: an obvious expansion of the periarterial lymphoid sheaths and a marked increase of the cells in red pulp within one week; a distinctive spread of necrotic loci on day 14. Conclusion This rat model of spleen transplantation has high success rate(100%). This method without stature vascular anastomosis technique can reduce operating time. This technique dosen't need clamping the systemic circulation. It is an ideal model and can be used for the experimental study related to spleen transplantation." @default.
- W2380480069 created "2016-06-24" @default.
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- W2380480069 date "2002-01-01" @default.
- W2380480069 modified "2023-09-25" @default.
- W2380480069 title "Establishment of model of heterotopic spleen transplantation in rats with stent and cuff technique" @default.
- W2380480069 hasPublicationYear "2002" @default.
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