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- W2380489039 abstract "[Objective] Antifungal substance from B. subtilis BS11 was studied to provide reference for further development and application.[Method]Growth conditions of B. subtilis BS11 in LB and NB broth were compared; effects of three methods for extracting antifungal substance from B. subtilis BS11 were compared; the optimal fermentation conditions of antifungal substance production were studied. [Result] Fusarium.oxysporum f. sp. Cubense was as the indicator,the B. subtilis BS11 had obvious difference in antifungal bioactivity and growth rate in LB and NA mediums,and it was stronger in NB broth than in LB broth,but grew faster in LB broth than in NB broth. Ethanol and ethyl acetate could not extract antifungal substances,and antifungal factor from BS11 was further identified as a kind disease-resistance protein by ammonium sulfate precipitation method. The optimums fermentation conditions of antifungal substance production were original p H7. 0,fermentation temperature 30 ℃and amount of load 60 ml( in 250 ml flask). The degrading test showed that the supernatant had protease activity and cellulase activity but didn't have chitinase activity. [Conclusion] The results provide theoretical basis for application of BS11 in controlling plant diseases." @default.
- W2380489039 created "2016-06-24" @default.
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- W2380489039 date "2014-01-01" @default.
- W2380489039 modified "2023-09-26" @default.
- W2380489039 title "Analysis on Antifungal Substance from Bacillus subtilis BS11" @default.
- W2380489039 hasPublicationYear "2014" @default.
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