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- W2380491377 abstract "The succinate-linked reduction of exogenous NAD~+ in submitochondrial particle preparation of rat liver has been studied.The submitochondrial particle preparations were capable of catalyzing the oxidation of β-hydroxybutyrate with coupled phosphorylation with a P/O ratio about 0.7.Exogenous NAD~+ was rapidly reduced by succinate in the presence of ATP.The energy-linked NAD~+ reduction was inhibited by amytal,dicumarol,and DNP~1),and was completely dependent on the presence of Mg~(++) or Mn~(++). However,higher concentrations of Mg~(++) or Mn~(++) have an inhibitory effect.The finding that the energy- linked NAD~+ reduction was inhibited by EDTA,even in the presence of an excessive amount of Mg~(++), indicates that the energy-linked NAD~+ reduction may require an additional metal ion other than free Mg~(++) or Mn~(++).Vitamin K_3 inhibited this energy-linked reduction of NAD~+ in the presence of cyanide. It seems unlikely that this inhibition resulted from increased aerobic reoxidation of NADH in the presence of vitamin K_3 as suggested by Ernster et al.It seems that vitamin K_3 acts by increasing the rate of hydro- lysis of high energy intermediates." @default.
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- W2380491377 date "1964-12-01" @default.
- W2380491377 modified "2023-09-25" @default.
- W2380491377 title "STUDIES ON OXIDATIVE PHOSPHORYLATION AND RELATED PROBLEMS. 3. THE SUCCINATE-LINKED REDUCTION OF EXOGENOUS NAD+ IN SUBMITOCHONDRIAL PARTICLES OF RAT-LIVER." @default.
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