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- W2380494746 abstract "Objective:To develop a high performance liquid chromatographic method for the determination of harpagide and harpagoside in Zengye Chengqi Koufuye.Method:High performance liquid chromatography was performed on an Eclipse XDB C18(4.6 mm×150 mm,5 μm)with acetonitrile-0.03% phosphoric acid with a gradient elution program as the mobile phase at a flow rate of 1.0 mL·min-1,210 nm as the detection wavelength,the temperature of column was at 30 ℃.Result:harpagide and harpagoside showed good relationship at the range of 0.257-1.284 μg(r=0.999 8,for harpagide),0.092-0.460 μg(r=1.000 0,for harpagoside),the average recoveries(n=5) were: 98.74%(RSD 0.58%),97.15%(RSD 1.02%).Conclusion:The results showed that the method is simple,accurate stable,and repeatable and it is suitable for quality control of Zengye Chengqi Koufuye." @default.
- W2380494746 created "2016-06-24" @default.
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- W2380494746 date "2012-01-01" @default.
- W2380494746 modified "2023-09-23" @default.
- W2380494746 title "Determination of Harpagide and Harpagoside in Zengye Chengqi Koufuye by HPLC" @default.
- W2380494746 hasPublicationYear "2012" @default.
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