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- W2380521729 abstract "In order to establish a single C.elegans multiplex PCR method to amplify specific DNA fragment and further exploring its application in preparing of nucleic acid molecular weight standards,six pair primers were designed according to the six chromosome DNA sequences of C.elegans and the MP primer and MFE primer programs were used to evaluate their specificities.Subsequently,a single nematode was used as the template to run multiplex PCR,followed with further identification by 20 g/L agarose gel electrophoresis.Different multiplex PCR products were obtained as expected.The six-primer multiplex PCR products are identical to the widely used nucleic acid molecular weight standard DL 2 000.Our method can be used to prepare different nucleic acid molecular weight standards.In summary,a fast and specific multiplex PCR method was developed and different nucleic acid molecular weight standards could be obtained by this method.This method provides a reference for further using of multiplex PCR." @default.
- W2380521729 created "2016-06-24" @default.
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- W2380521729 date "2012-01-01" @default.
- W2380521729 modified "2023-09-25" @default.
- W2380521729 title "A Method for Rapid Preparation of DNA Molecular Weight Standard by Single C. elegans Multiplex PCR" @default.
- W2380521729 hasPublicationYear "2012" @default.
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