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- W2380538213 abstract "Objective To obtain human antibody against human interferon alpha(huIFN-α) from human synthetic recombinant antibody libraryfor the therapy of systemic lupus erythematosus. Methods In our study, using phage display technology, antibodies against purified huIFN-α1b and huIFN-α2b were screening from a full synthetic human phage display library. Antibody genes were identified by sequence analysis and their stability and specificity of antibodies confirmed several times by phage ELISA. The scFv antibody genes were cloned into vector pET22b and expressed in E. coli strain BL21(DE3), and secreted antibodies were further detected by Western blot assay. After purified by metal chelate affinity chromatography (MCAC), the scFv antibodies were further detected by Western blot assay. Results After three rounds of panning, 100 human scFv clones with specific reactivity with huIFN-α1b but not huIFN-α2b and huIFN-γ were selected by phage ELISA. Nine different antibody genes were identified by sequence analysis of 86 clones. And 7 antibody genes were derived from VH3 and VL3 germline family and 2 antibody genes derived from VH3 and VL1 germline family. Five of 9 phage antibodies were found to bind huIFN-α1b with a high stability and specificity by phage ELISA. Three of 5 secreted single chain antibodies were found to bind huIFN-α1b with a high specificity by ELISA test. The purified scFv antibodies were detected to bind huIFN-α1b without cross-reaction by Western blot assay. Conclusion Three single chain antibodies against human interferon alpha (huIFN-α) with a high stability and specificity were obtained from human synthetic recombinant antibody library." @default.
- W2380538213 created "2016-06-24" @default.
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- W2380538213 date "2009-01-01" @default.
- W2380538213 modified "2023-09-26" @default.
- W2380538213 title "Antibody against human interferon α1b from human synthetic recombinant antibody library" @default.
- W2380538213 hasPublicationYear "2009" @default.
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