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- W2380596148 abstract "Rehmannia,a genus of Scrophulariaceae,is widely distributed in central,eastern and northern China. However, it is relatively difficult to distinguish species by traditional morphological methods due to rapid speciation of the genus. Recently developed DNA barcoding provides a new approach to identify species quickly and accurately. In this study,three candidate DNA noncoding regions(trn L-trn F, trn M-trn V and trn S-trn G) from the chloroplast genome and internal transcribed spacer(ITS) region from the nuclear genome were evaluated among 75 individuals of five species of Rehmannia,and identification efficiency was assessed using PWG-distance and Tree-Building methods. Results indicated that the five Rehmannia species could not be accurately distinguished by single or the combination of chloroplast DNA fragments with low species discrimination rates(0%- 20%),but could by the combination of chloroplasts and nuclear DNA regions(trn S-trn G + ITS) with 100%discrimination rates. Thus, trn S-trn G + ITS can be considered as a potential barcode for species identification in Rehmannia. The combination of chloroplasts and nuclear fragments can be widely adopted for the discrimination of plant species when a single DNA barcode fails." @default.
- W2380596148 created "2016-06-24" @default.
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- W2380596148 date "2015-01-01" @default.
- W2380596148 modified "2023-09-23" @default.
- W2380596148 title "DNA Barcoding of the Genus Rehmannia(Scrophulariaceae)" @default.
- W2380596148 hasPublicationYear "2015" @default.
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