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- W2380596637 abstract "【Objective】Real time PCR was established in Didymella bryoniae. 【Method】According to published sequences in Genebank,the gene beta- tubulin( tub2) was chosen as the target gene. The specific primer and Taq Man probe were designed according to beta- tubulin( tub2) gene sequence in D. bryoniae. The reaction conditions were optimized. 【Result】The primers and probe were highly sensitive by detecting other strains in melon and similar strains. The sensitivity could reach to 356 fg / μL by real time PCR. The sensitivity was ten times than that of general PCR.【Conclusion】The primers and Taqman probe were specific by detecting infected plants and unknown samples." @default.
- W2380596637 created "2016-06-24" @default.
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- W2380596637 date "2015-01-01" @default.
- W2380596637 modified "2023-09-23" @default.
- W2380596637 title "Study on the Method of Detecting the Didymella Bryoniae by Real- Time PCR" @default.
- W2380596637 hasPublicationYear "2015" @default.
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