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- W2380637526 abstract "Aim To construct the eucaryotic recombi nant plasmid con-taining the chimaeric E gene from the Chinese dengue type 2and 4viruses,analyze the immunogenicit y of the recombinant plasmid DNA,and contribute to the study on multiv alent dengue vaccines.Methods The chimaeric E gene fragment contai ns the domains I /II of D2-43strain and domain III of D4-B5strain,and was cloned into the eu-caryotic plasmid pcDNA3.1.The sequ ence correctness of the chimaeric gene was affirmed by nucleotide sequencing.Then,Balb /C mice were immunized by the recombinant plasmi d with i.m ,and the specific an-tibodies to dengue viruses in the pooled sera were detected by IFA.Results The sequences of the chimaeric E gene contained in the recom-binant plasmid,named pcDNA -D2/4,w ere determined and showed to be exactly the same as the correspond ing parts of D2-43and D4-B5.Three weeks after the first immuniza tion,specific fluorescence was de-tected to both D2-43and D4-B5strain s.Conclusion By immunizing mice with recombinant eucaryotic ex pression plasmid containing the chi -maeric E gene from D2-43and D4-B5,sp ecific antibodies to both viruses were induced simultaneously,which conduces to the study on multivalent dengue vaccines." @default.
- W2380637526 created "2016-06-24" @default.
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- W2380637526 date "2001-01-01" @default.
- W2380637526 modified "2023-09-27" @default.
- W2380637526 title "Study of the immunogenicity of the chimaeric E gene from Chinese Dengue 2 and 4viruses" @default.
- W2380637526 hasPublicationYear "2001" @default.
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