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- W2380662208 abstract "cDNA-AFLP was a powerful tool for isolating differential expressed genes in plants,and has been widely applied to the research fields of plant resistance,growth and development. In this paper,Some differential expressed fragments were selected between the BYDV resistant wheat line HW642 and its susceptible wheat parent Zhong8601 selected through cDNA-AFLP technique in order to identify some related defense genes against BYDV in the resistant wheat lines. Results showed that 6 differential expressed fragments via cDNA-AFLP were confirmed by reversed Northern blot,and could be involved in defense response to BYDV in the resistant wheat line HW642; 3 differential expressed fragments via cDNA-AFLP were confirmed by the extension re-amplified/PAGE electrophoresis method and RT-PCR analysis. These confirming methods should be suitable to identify the ture differential expressed fragments selected via cDNA-AFLP method." @default.
- W2380662208 created "2016-06-24" @default.
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- W2380662208 date "2009-01-01" @default.
- W2380662208 modified "2023-09-27" @default.
- W2380662208 title "Confirmation of cDNA-AFLP Differential Expressed Fragments of Related Defense Genes agaist BYDV in Wheat" @default.
- W2380662208 hasPublicationYear "2009" @default.
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