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- W2380666329 abstract "The transgenic yeast Pichia pastoris cultured for high level expression of rHSA was fed with Na_2SeO_3 during the induction phase in order to investigate incorporation of selenium into rHSA.After fermentation,the supernatant was processed by heat treatment,ultrafiltration,ion exchange chromatography and acetone precipitation and the molecular weight determined by Tricine-SDS-PAGE.Energy dispersive X-ray analysis(EDX) and atomic absorption spectroscopy(AAS) were then used to determine the selenium concentration.The results indicated that Na_2SeO_3 directly affects cell growth and rHSA yield.Furthermore,the experimental sample is found to contain 23.2% more selenium than the control(no addition of Na_2SeO_3) and the ratio of selenium to rHSA is 0.0069,which is about 35 times higher than the control." @default.
- W2380666329 created "2016-06-24" @default.
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- W2380666329 date "2006-01-01" @default.
- W2380666329 modified "2023-09-26" @default.
- W2380666329 title "Selenium incorporation into recombinant human serum albumin in Pichia pastoris" @default.
- W2380666329 hasPublicationYear "2006" @default.
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