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- W2380681337 abstract "Objective To explore the culture conditions for the neural stem cells from embryonic rat cortices and to test their efficacy of expressing exogenous gene. Methods The cells from the embryonic rat cortices were mechanically dissociated and the N2 medium was adopted to culture and expand the cells. Then the cells were identified by immunocytochemistry method, and the Ad5βgal vector was applied to test their capacity of expressing foreign gene. Results The neural stem cells from embryonic rats were successfully cultured, which formed typical neurospheres in suspension and expressed nestin and vimentin. Most cells were infected by Ad5βgal gene and expressed lacZ gene. The cells could be passaged by mechanical method, frozen and thawed by general procedure, they could be passaged and expanded nearly for up to 3 months. Conclusion The neural stem cells can be cultured and passaged steadily in vitro. They can express βgal foreign gene and are the ideal vectors for cell therapy and gene therapy." @default.
- W2380681337 created "2016-06-24" @default.
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- W2380681337 date "2002-01-01" @default.
- W2380681337 modified "2023-09-25" @default.
- W2380681337 title "The long-term culture in vitro and exogenous gene expression of neural stem cells" @default.
- W2380681337 hasPublicationYear "2002" @default.
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