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- W2380685717 abstract "Objective:To use high performance liquid chromatography(HPLC) method for determination of basic orange in paprika.Methods: Samples were weighed in 50 ml plastic centrifuge tube with 10 ml extraction.After ultrasonication and centrifugation,the supernatant was taken for filtration through the membrane and sample injection.The separations were performed with an XDB-C18 column(5 μm,4.6×250 mm) at flow rate of 1.0 ml/min.The mobile phase was acetonitrile-ammonium acetate(75+25).The diode array detector was applied.Results: The basic orange showed good linearity in the range of 1.0 μg/ml～50.0 μg/ml(r=0.9992) and better peak shape.The recoveries were 93.4% and the RSD was 0.61%.The detection limit was 0.3 μg/ml.Conclusion: The method for determination of basic orange by HPLC in paprika have the advantages of qualitative accuracy,high sensitivity,good recovery and reproducibility,fast operation,accurate and reliable results." @default.
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- W2380685717 date "2012-01-01" @default.
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- W2380685717 title "HPLC method for the determination of basic orange in paprika" @default.
- W2380685717 hasPublicationYear "2012" @default.
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