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- W2380694693 abstract "We provide a modified and simple method to isolate the total RNA from basidiomycetes Volvariella volvacea The purified RNA is ready for use in standard and downstream applications such as RT PCR, poly A RNA selection, differential display, Northern dot and slot blotting, primer extension, cDNA synthesis, expression array and expression chip analysis We have successfully isolated total RNA from Volvariella volvacea with the way for many times The value of A 260 / A 280 fell in the range from 1 7 to 2 0 and that of A 260 / A 230 is larger than 2 0 which indicated that the purity of RNA was satisfactorily high (RNA was not contaminated by proteins or benzene phenol etc ) Electrophoresis analyzing total RNA sample show total RNA hasn't been broken down because 28S∶18S is equal to 2∶1" @default.
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- W2380694693 date "2004-01-01" @default.
- W2380694693 modified "2023-09-27" @default.
- W2380694693 title "Rapid isolation of total RNA from basidomycetes Volvariella volvacea" @default.
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