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- W2380701334 abstract "In order to establish a stable ISSR-PCR reaction system for pitaya,a single factor experiment and orthogonal method was applied for the optimization.The results showed that the optimum concentrations of different components in 20 μL reaction system were 2.0 μL 10×Buffer(with Mg2+),20.0 ng template DNA,0.30 mmol·L-1 dNTP,0.35 μmol·L-1 primer and 1.5 U Taq DNA polymerase.The optimized annealing temperature was 54.0℃ for the primer(AC)8T.The established ISSR-PCR system was tested on 6 pitaya samples with satisfactory performance." @default.
- W2380701334 created "2016-06-24" @default.
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- W2380701334 date "2010-01-01" @default.
- W2380701334 modified "2023-09-24" @default.
- W2380701334 title "Optimization of ISSR reaetion system in pitaya" @default.
- W2380701334 hasPublicationYear "2010" @default.
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