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- W2380734803 abstract "Objective To detect and analyze the virus infection status among laboratory animals in Hunan Province.Methods A total of 56 rats and 135 Balb/c mice were randomly collected from the laboratory animal production and use units in Hunan Province.The antibodies of rat hantannvirus,rat sendai virus,mouse sendai virus,mouse poxvirus and mouse hepatitis virus were detected by ELISA.The nucleic acids of enterovirus 71,coxsackievirus A16 and rotavirus were detected by real-time PCR.The hantannvirus M gene was determined by PCR after specific primers were designed and the positive product was sequenced and analyzed by bioinformatics methods to determine the genetype of the virus.Results The antibodies of hantannvirus and sendai virus were not detected in 56 serum samples of rats,and the antibodies of mouse sendai virus,poxvirus and hepatitis virus were not found in 135 serum samples of mice.189 blood samples from rats and mice showed negative results for nucleic acids of enterovirus 71,coxsackievirus A16 and rotavirus.One sample of SD rat was positive for hantannvirus Mgene and the product size was about 860 bp.BLAST analysis confirmed that the gene fragment belonged to hantannvirus.Further phylogenetic analysis showed it belonged to the HTN subtype of hantannvirus,and when compared to the vaccine strain Z10,the nucleotide homology and amino acid homology were 86% and 89% respectively.Conclusions The laboratory animals in Hunan Province have hantannvirus infection and should be timely immunized.Laboratory animal practitioners should receive health examination regularly." @default.
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- W2380734803 date "2014-01-01" @default.
- W2380734803 modified "2023-09-27" @default.
- W2380734803 title "Analysis of the virus infection status in laboratory animals in Hunan Province" @default.
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