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- W2380762022 abstract "Objective Our purpose was to found a technological process for the fast isolation and purification of the defibrase from the venom of Agkistrodon acutus Methods Preliminary isolated by the complexing with EDTA and precipitating with potassium thiocyanate,and the active fraction was further purified by the DEAE Sepharose Fast Flow and sephadex 75 prep grade chromatagraphy,Finally the defibrase components acorresponding with the new national standards were obtained Results Ninty two percents of hemorrhagin and 65 17% fractions without Thromin like enzyme(TLE) activity were eliminated in the preliminary isolation,and the activity of TLE been recovered up to 92 68% there were at least four kinds of TLE,amony them one being the defibrase in the chromatography fractions The activity of the defibrase obtained by these process was more then 1 200 Bu/mg,and up to 1 503 Bu/mg,and the output of defibrase from one gram crystal venom of Agkistrodon acutus could be up to 2998 116 Bu,the purity of the product was high:SDS Page:only one protein band locating nearby the protein marker with M W 36 000Da;HPLC:Single peak Conclusion The technological process for defibrase isolation and purification introduced in this paper is fast and efficient enough to be adapted to manufacturing scale [" @default.
- W2380762022 created "2016-06-24" @default.
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- W2380762022 date "2000-01-01" @default.
- W2380762022 modified "2023-09-25" @default.
- W2380762022 title "Fast Isolation and Purification of the Defibrase from Crystal Venom of Agkistrodon acutus" @default.
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