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- W2380771252 abstract "The r DNA complete sequence of tobacco anthracnose pathogen was cloned and sequenced. The full length of the r DNA sequence is 2870 bp. Sequence comparison results showed that the rDNA of tobacco anthracnose pathogen shared a high sequence similarity(96.0%-96.2%) with that of Colletotrichum gloeosporioides. Furthermore, from the constructed phylogenetic tree, it was indicated that tobacco anthracnose pathogen and C. gloeosporioides clustered into a separate branch, which indicated that tobacco anthracnose pathogen had the closest relationship with C. gloeosporioides. Therefore, combined with its morphology characteristic, tobacco anthracnose pathogen isolated from tobacco plant in Guizhou could be preliminarily identified as C. gloeosporioides. A pair of specific primer was designed according to the rDNA sequence of tobacco anthracnose pathogen. Not only tobacco anthracnose pathogen could be identified from 8 different pathogens isolated from tobacco plants, but also it could be identified from 7 different anthracnose pathogens. Plants of Nicotiana tabacum were inoculated with tobacco anthracnose pathogen. Using the total DNA extracted from the diseased tissue as a template, a specific band could be amplified by PCR with the specific primer. It was suggested that the method could be applied for the identification and rapid detection of C. gloeosporioides from tobacco plants." @default.
- W2380771252 created "2016-06-24" @default.
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- W2380771252 date "2015-01-01" @default.
- W2380771252 modified "2023-09-23" @default.
- W2380771252 title "Molecular identification and detection of tobacco anthracnose pathogen" @default.
- W2380771252 hasPublicationYear "2015" @default.
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