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- W2380779999 abstract "Cloning by PCR and sequencing of ARC1Boler gene from genomic DNA and stigma cDNA in Brassica oleracea L. of self-incompatibility are immensely important. It helps to understand of ARC1 gene mediating in self-incompatibility signaling process. In this paper, ARC1Boler gene was amplified and sequenced by PCR with template genomic DNA and cDNA from roots, leaves, petals, stigma and ovaries during bud stage. The major results are as follows: ARC1Boler gene was steadily amplified from genomic DNA and stigma cDNA, but there was no PCR products using cDNA of roots, leaves, petals and ovaries as templates, which suggested that ARC1Boler gene was expressed only in the stigma. ARC1Boler gene showed 94% identity to the sequence of ARC1 gene of Brassica napus L. There was only a 1 983 bp ORF in ARC1Boler gene which encodes 661 amino acids. The sequence of its amino acids showed 85% and 52% identity to those of ARC1Bnap gene and ARC1At gene respectively. In three proteins, there were two structures, Ubox and Arm Repeats. Ubox was possibly the domain of dimeration of ARC1 protein. In the C-terminal of ARC1Boler protein and ARC1Bnap protein, there were five arm repeats, while only one in ARC1At protein. This difference may potentially determine the discrepancy of function of ARC1Boler, ARC1Bnap and ARC1At. In ARC1Boler protein and ARC1Bnap protein, the arm repeats of C-terminal are conceivably conservative domain." @default.
- W2380779999 created "2016-06-24" @default.
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- W2380779999 date "2004-01-01" @default.
- W2380779999 modified "2023-09-25" @default.
- W2380779999 title "Cloning and Characterization of Encoding Sequence of SRK-Binding Protein ARC1 from Brassica oleracea L. in Self-incompatibility Signaling Process" @default.
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