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- W2380803326 abstract "Objective To understand the regulation of interleukin-13 receptor α2 gene transcription in the pathogenesis of bronchial asthma.Methods Various lengths of gene segments(F1～F6)of promoter region in hIL-13Rα2 gene was amplified by PCR.F1 and F2 contained signal transducer and transcription activator 6 (STAT6) binding domain.The amplified DNA fragments were TA-cloned into pGEM-Teasy vector.After fluorescence and PCR screenings,suitable clones were selected to extract plasmid DNA and restriction enzyme digestion analysis were carried out.To exclude clones of mismatching during PCR amplification,the sequences of all clones were determined.The inserts of TA-clone were subcloned into pGL3 enhancer vector.Plasmids were extracted from these constructs and then were confirmed through restriction enzyme digestion analysis.Proper clones were selected for extracting plasmid on a large scale.pGL3 vector constructs (F1～F6 and mock) were transfected into mouse bronchial epithelial cell line TGMBE-02-3.After 24 hours,mIL-4 (10 μg/L ) or mIL-13 (50 μg/L) was added.Luciferase assay was performed 24 hours after stimulation.Results (1)The sequence of hIL-13Rα2 promoter region from genomic DNA (BCI001) was identical to the sequence from Gene Bank.(2) After transfection of all constructs,the relative fluroscence activities had no significant difference between control group and mIL-4/mIL-13 stimulation group.Conclusion mIL-4 or mIL-13 stimulation did not affect the transcriptional activity of hIL-13Rα2 gene." @default.
- W2380803326 created "2016-06-24" @default.
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- W2380803326 date "2004-01-01" @default.
- W2380803326 modified "2023-09-25" @default.
- W2380803326 title "Regulation of IL-13Rα2 transcription in mouse bronchial epithelial cells induced by IL-4/IL-13" @default.
- W2380803326 hasPublicationYear "2004" @default.
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