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- W2380845726 abstract "Objective To investigate the sensitivity and feasibility of K-ras gene detected by direct sequencing.Methods Using the certified cell colons containing wild and mutant type K-ras gene,the gradient concentrations were got,the mutation of samples were tested by the direct sequencing method,the sensitivity of the experiment was estimated.Results Both the forword and reverse sequencing showed that each of the five tubes which had the 5% mutant DNA showed no K-ras mutation,and all the tubes which contain 10% mutant DNA showed K-ras mutation.The ratio of mutant base T and wild base G had statistic difference in every groups(all P0.05).The height of the mutant peak was increased with the concentration of the mutant DNA,there was definite correlation between them(r=-1.00,Sig.0.05).Conclusion Using the sensitivity of 10%,the specificity of DNA direct sequencing could reach 100%.The height of the mutant peak of the electrophoretogram could be used to half-quantify the concentration of the mutant DNA in the sample,and which was satisfactory for clinical test and study." @default.
- W2380845726 created "2016-06-24" @default.
- W2380845726 creator A5027370151 @default.
- W2380845726 date "2011-01-01" @default.
- W2380845726 modified "2023-09-26" @default.
- W2380845726 title "Sensitivity confirmation and assessment of the direct sequencing for the K-RAS gene in colorectal cancers" @default.
- W2380845726 hasPublicationYear "2011" @default.
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