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- W2380870768 abstract "Background and purpose:The membrane cytoskeleton crosslinker Ezrin participates in several functions including cell adhesion, motility and cell survival, and there is increasing evidence that it regulates tumor progression. However, the role played by Ezrin in osteosarcoma has not been clearly delineated. The purpose of this experiment was to investigate the effect of membrane-cytoskeleton linker Ezrin on the proliferation and invasion of human osteosarcoma.Methods:Human osteosarcoma cell line MG -63 was cultured. A plasmid of a short hairpin RNA targeting Ezrin was constructed and transfected into MG-63 cell line. The expression of Ezrin mRNA and protein were examined by RT-PCR and Western blot method respectively. The proliferation was assayed by MTT. Flow cytometry was used to detect the cell cycle. Transwell test was used to detect the invasion ability of MG-63.Results:RT-PCR revealed that Ezrin shRNA notably down-regulated expression at mRNA levels. Western blot also revealed notably down-regulated expression at protein levels. The proliferation was inhibited after RNAi treatment. The cell proportion in G2-M phase decreased from 25.05% to 18.36%. The invasion ability decreased from 35.9±5.6 to 22.5±2.8(P0.01).Conclusions:Ezrin is necessary for human osteosarcoma cell proliferation and invasion. It is probably a potential target for osteosarcoma treatment." @default.
- W2380870768 created "2016-06-24" @default.
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- W2380870768 date "2008-01-01" @default.
- W2380870768 modified "2023-09-25" @default.
- W2380870768 title "The influence of down-regulation of Ezrin expression by short hairpin RNA on the proliferation and invasion of osteosarcoma" @default.
- W2380870768 hasPublicationYear "2008" @default.
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