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- W2380879730 abstract "Wheat landrace Hongyoumai carries one dominant resistance gene for powdery mildew (Blumeria graminis), tentatively designated Pmhym. To further elucidate molecular mechanism of the resistance to powdery mildew in Hongyoumai, a resistant pool was constructed with four homogenous resistant lines from the F3 generation of Yumai 13/Hongyoumai. The wheat seed- lings were inoculated with single spore isolates of B. graminis f. sp. tritici, and at 0 and 24 h after inoculation the gene expression profile was analyzed using Affymetrix wheat microarray. There were approximately 5 282 expressed genes (with log ratio ≥ 2 or ≤ 0.5) among total 61 127 genes set in the microarray plate, including 2 553 up-regulated and 2 729 down-regulated genes or expressed sequence Tags (ESTs). In the up-regulated ESTs whose functions have been known, 39.81% were disease/defense genes. In the down-regulated ESTs, energy genes and disease/defense genes accounted for 26.71% and 19.65%, respectively. A total of 81 ESTs were up-regulated more than eight times, in which most were related to disease resistance, such as pathogenesis-related protein, defense genes, genes producing or eliminating reactive oxygen species, and genes involved in signal transduction. Quan-titative RT-PCR (qRT-PCR) was carried out with 13 probe sets up-regulated and down-regulated more than eight times which validated a good reproducibility of microarray analysis." @default.
- W2380879730 created "2016-06-24" @default.
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- W2380879730 date "2009-01-01" @default.
- W2380879730 modified "2023-09-23" @default.
- W2380879730 title "Expression Profile of Landrace Hongyoumai Infected by Blumeria graminis f. sp. tritici Using Gene Microarray" @default.
- W2380879730 hasPublicationYear "2009" @default.
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