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- W2380886968 abstract "AIM: To establish a quantitative system to characterize different RNA molecules (transcripts) of hepatitis B virus (HBV) in circulation. METHODS: Viral nucleic acids were extracted from serum samples and HBV DNA and RNA were characterized quantitatively by competitive PCR and RT-PCR procedures. RESULTS: A seroassay was established to characterize 3′-end structure of the full-length and truncated viral transcripts. Copy numbers of viral RNA/DNA segments corresponding to X, Core and X Precore regions were determined, representing early, middle and late stages of HBV gene replication, respectively. In addition, the data demonstrate dynamic changes in the circulating viral transcripts as well as DNA in their copy numbers and structures during lamivudine therapy. After a 8-week treatment, the copy numbers for C or PreC/X DNA segments decreased to 10 5·mL -1 from 10 9·mL -1 , significantly below that for X segment (from 10 9·mL -1 to 10 7·mL -1 ). There was no significant decrease in RNA copy numbers. Polyadenylated HBV RNA was also determined using anchored oligo (dT) primers targeting fRNA and trRNA . The copy numbers of fRNA and trRNA were 10 5 copies per mL of serum during most of the treatment period, significantly below that of X segment (10 7·mL -1 ). The excess of X segment RNA over fRNA levels suggested a packaging related removal of poly (A) 3′ ends. CONCLUSION: An assay for the detection of copy numbers and different 3′end structures of the circulating HBV transcripts is established, which provides a more precise approach to the detection of dynamic change of circulating HBV transcripts." @default.
- W2380886968 created "2016-06-24" @default.
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- W2380886968 date "2003-01-01" @default.
- W2380886968 modified "2023-09-25" @default.
- W2380886968 title "Quantitative assay system established for different hepatitis B virus RNAs in sera sample" @default.
- W2380886968 hasPublicationYear "2003" @default.
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