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- W2380908088 abstract "FMDV non-structural protein gene(NSPs) 3ABC, 3AB, 2C and 3D were amplified and cloned into expression plasmid pET-32a(+). The recombinant NSPs were produced in E.coli and purified using Ni~(2+) affinity column. Western-blotting indicated that the NSPs were expressed correctly. Using the recombinant NSPs, indirect ELISAs have been set up to distinguish FMDV-infected pigs from vaccinated ones. Experimental results indicate that the immunogenesity of recombinant 3AB protein is strong and can be the ideal antigen for detection; the immunogenecity of 2C is weak and sensitivity of the assay is low; 3D is not an ideal antigen ,for the specificity of assay based on 3D is low." @default.
- W2380908088 created "2016-06-24" @default.
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- W2380908088 date "2005-01-01" @default.
- W2380908088 modified "2023-09-23" @default.
- W2380908088 title "Expression and Application of the Recombinant Non-Structural Proteins of FMDV" @default.
- W2380908088 hasPublicationYear "2005" @default.
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