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- W2380911066 abstract "To study the effect of RNAi on MC1Rexpression in mouse embryo fibroblasts(MEF). MC1Rgene was taken as candidate gene affecting the formation of mammal's hair color.The aim of the study was to inhibit the expression of MC1Rby RNA interference in the MEF.Three pairs of specific siRNA for MC1Rgene were designed for RNA interference and transferred into the MEF by reverse transfection.After 48h,the total RNA and protein were extracted from the MEF,and the relative expression of MC1RmRNA and protein was detected by using QRT-PCR technology and Western bolt.The results showed that the relative expression of MC1R mRNA and MC1Rprotein in the siRNA1and siRNA2groups were significantly lower(P0.05)than those in the blank control group,but the expression of siRNA3group was unconspicuous.These tests were successfully inhibited the expression of MC1Rin the MEF by RNA interference(reverse transfection)with LipofectamineTM RNAi-Mate.The expression trendency of MC1Rgene and protein are consistent.The results showed that the inhibition effect of siRNA1and siRNA2 groups were effective,the effective siRNA were screened from three specific siRNAs,can be served as a scientific foundation for the further study of regulation mechanism of MC1Rin the process of coat development." @default.
- W2380911066 created "2016-06-24" @default.
- W2380911066 creator A5007148727 @default.
- W2380911066 date "2014-01-01" @default.
- W2380911066 modified "2023-09-23" @default.
- W2380911066 title "Effect of RNAi on MC1R Expression in Mouse Embryo Fibroblasts" @default.
- W2380911066 hasPublicationYear "2014" @default.
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