Matches in SemOpenAlex for { <https://semopenalex.org/work/W2380921978> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2380921978 endingPage "1427" @default.
- W2380921978 startingPage "1420" @default.
- W2380921978 abstract "Essentials Nonacog beta pegol (N9-GP) activity is overestimated in clot method using silica-based reagents. Mimicking contact activation phase with silica reveals N9-GP activation before recalcification. Localization of N9-GP to silica facilitates activation by factor XIa and plasma kallikrein. Silica-based reagents to be used with caution when monitoring N9-GP therapy using clot method.Background Clinical laboratories routinely quantify factor IX (FIX) activity by measurement of the activated partial thromboplastin time (APTT) in a one-stage (OS) clotting assay. This assay can be performed with any of a plethora of differently composed APTT reagents, giving variable recovery when applied to nonacog beta pegol (N9-GP), an N-glycoPEGylated recombinant FIX. Objective To identify the cause of observed overestimations of N9-GP activity in an OS FIX clotting assay when most APTT reagents containing silica are used as the contact activator, and to elucidate the underlying mechanism. Methods Experiments mimicking the contact activation and clotting phases of the OS assay, combined with the use of plasmas with various deficiencies, were employed to shed light on the unique behavior of N9-GP. Confirmatory activations of N9-GP with purified enzymes and physical adsorption to silica particles were studied, and the influence of free polyethylene glycol (PEG) on these processes was investigated. Results N9-GP, but not native FIX, added to FIX-deficient plasma was prematurely converted to activated FIX (FIXa) during the contact activation phase of the clotting assay. Activated FXI (FXIa) and plasma kallikrein (PK) were responsible for the activation of N9-GP, an event that appeared to require the presence of a silica-containing APTT reagent. PEG-dependent adsorption of N9-GP to silica particles could be demonstrated. Conclusions The PEG moiety mediates colocalization of N9-GP with its activators FXIa and PK on silica surfaces, thereby facilitating premature conversion of N9-GP to FIXa during the contact activation phase, and leading to overestimation of the FIX activity in the OS clotting assay." @default.
- W2380921978 created "2016-06-24" @default.
- W2380921978 creator A5004228898 @default.
- W2380921978 creator A5044364194 @default.
- W2380921978 creator A5057311720 @default.
- W2380921978 creator A5081018170 @default.
- W2380921978 date "2016-07-01" @default.
- W2380921978 modified "2023-10-18" @default.
- W2380921978 title "Overestimation of N‐glycoPEGylated factor IX activity in a one‐stage factor IX clotting assay owing to silica‐mediated premature conversion to activated factor IX" @default.
- W2380921978 cites W2002307029 @default.
- W2380921978 cites W2005719363 @default.
- W2380921978 cites W2007420660 @default.
- W2380921978 cites W2007881102 @default.
- W2380921978 cites W2009052421 @default.
- W2380921978 cites W2019166532 @default.
- W2380921978 cites W2044693511 @default.
- W2380921978 cites W2063937984 @default.
- W2380921978 cites W2069900144 @default.
- W2380921978 cites W2071038033 @default.
- W2380921978 cites W2151410451 @default.
- W2380921978 doi "https://doi.org/10.1111/jth.13359" @default.
- W2380921978 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27169618" @default.
- W2380921978 hasPublicationYear "2016" @default.
- W2380921978 type Work @default.
- W2380921978 sameAs 2380921978 @default.
- W2380921978 citedByCount "42" @default.
- W2380921978 countsByYear W23809219782016 @default.
- W2380921978 countsByYear W23809219782017 @default.
- W2380921978 countsByYear W23809219782018 @default.
- W2380921978 countsByYear W23809219782019 @default.
- W2380921978 countsByYear W23809219782020 @default.
- W2380921978 countsByYear W23809219782021 @default.
- W2380921978 countsByYear W23809219782022 @default.
- W2380921978 countsByYear W23809219782023 @default.
- W2380921978 crossrefType "journal-article" @default.
- W2380921978 hasAuthorship W2380921978A5004228898 @default.
- W2380921978 hasAuthorship W2380921978A5044364194 @default.
- W2380921978 hasAuthorship W2380921978A5057311720 @default.
- W2380921978 hasAuthorship W2380921978A5081018170 @default.
- W2380921978 hasConcept C126322002 @default.
- W2380921978 hasConcept C152328610 @default.
- W2380921978 hasConcept C178790620 @default.
- W2380921978 hasConcept C181199279 @default.
- W2380921978 hasConcept C185592680 @default.
- W2380921978 hasConcept C2778261982 @default.
- W2380921978 hasConcept C2778382381 @default.
- W2380921978 hasConcept C2781221834 @default.
- W2380921978 hasConcept C2992139802 @default.
- W2380921978 hasConcept C40875361 @default.
- W2380921978 hasConcept C43617362 @default.
- W2380921978 hasConcept C52853521 @default.
- W2380921978 hasConcept C55493867 @default.
- W2380921978 hasConcept C71924100 @default.
- W2380921978 hasConcept C77171857 @default.
- W2380921978 hasConceptScore W2380921978C126322002 @default.
- W2380921978 hasConceptScore W2380921978C152328610 @default.
- W2380921978 hasConceptScore W2380921978C178790620 @default.
- W2380921978 hasConceptScore W2380921978C181199279 @default.
- W2380921978 hasConceptScore W2380921978C185592680 @default.
- W2380921978 hasConceptScore W2380921978C2778261982 @default.
- W2380921978 hasConceptScore W2380921978C2778382381 @default.
- W2380921978 hasConceptScore W2380921978C2781221834 @default.
- W2380921978 hasConceptScore W2380921978C2992139802 @default.
- W2380921978 hasConceptScore W2380921978C40875361 @default.
- W2380921978 hasConceptScore W2380921978C43617362 @default.
- W2380921978 hasConceptScore W2380921978C52853521 @default.
- W2380921978 hasConceptScore W2380921978C55493867 @default.
- W2380921978 hasConceptScore W2380921978C71924100 @default.
- W2380921978 hasConceptScore W2380921978C77171857 @default.
- W2380921978 hasIssue "7" @default.
- W2380921978 hasLocation W23809219781 @default.
- W2380921978 hasLocation W23809219782 @default.
- W2380921978 hasOpenAccess W2380921978 @default.
- W2380921978 hasPrimaryLocation W23809219781 @default.
- W2380921978 hasRelatedWork W1436712773 @default.
- W2380921978 hasRelatedWork W1982170684 @default.
- W2380921978 hasRelatedWork W199796774 @default.
- W2380921978 hasRelatedWork W2071937323 @default.
- W2380921978 hasRelatedWork W2084973082 @default.
- W2380921978 hasRelatedWork W2175700465 @default.
- W2380921978 hasRelatedWork W2323744759 @default.
- W2380921978 hasRelatedWork W2468319422 @default.
- W2380921978 hasRelatedWork W4255403527 @default.
- W2380921978 hasRelatedWork W623533348 @default.
- W2380921978 hasVolume "14" @default.
- W2380921978 isParatext "false" @default.
- W2380921978 isRetracted "false" @default.
- W2380921978 magId "2380921978" @default.
- W2380921978 workType "article" @default.