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- W2380938888 abstract "The 16S rRNA gene-based PCR has been developed as a routine method for phytoplasma detection. When P1/P7 (or U5/P7) nested U3/U5 primers were used in PCR,the normal 0.85 kb product band was often accompanied by an unexpected 0.36 kb fragment. The occurrence and intensity of this extra band was tightly related to the normal band. This phenomenon was only observed in the nested-PCR detection using P1/P7 (or U5/P7) nested U3/U5 primers,and never occurred in routine PCR using P1/P7,U5/P7 or U3/U5 primer pairs. So far,the extra PCR band was found at least in grapevine yellows (stolbur) and elm yellows phytoplasma detection. Further PCR amplification and nucleotide analysis revealed that this extra fragment located on phytoplasma 16S rRNA gene with a partial complement site of U5 primer. These results revealed the potential usage of this extra band in phytoplasma detection." @default.
- W2380938888 created "2016-06-24" @default.
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- W2380938888 date "2005-01-01" @default.
- W2380938888 modified "2023-09-25" @default.
- W2380938888 title "Analysis of an extra fragment amplified by P1/P7 nested U3/U5 primers in grapevine yellows (stolbur) phytoplasma detection" @default.
- W2380938888 hasPublicationYear "2005" @default.
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