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- W2380966032 abstract "Objective:To gain the recombinant MPT64protein of Mycobacterium tuberculosis,study its immuno-logical characteristics and to evaluate its potential value for TB serodiagnosis.Methods:DNA fragments code for the protein were obtained by PCR,then cloned into the expression vector pET-9d to gain recombinant E.coli BL21(DE3).The expressed protein analyzed by SDS-PAGE,and purified by two anion exchange columns.The immuno-logical characteristics of the recombinant MPT64were analyzed by Western-blotting and its serodiagnostic value de-tected by ELISA.Results:The clone was found to produce23kDa protein similar to the molecular weight of natural MPT64Protein.Analyzed by SDS-PAGE,the recombinant MPT64protein existed in the form of solution in E.coli.and amounted to30%of total cell proteins.ELISA results indicated that the purified recombinant protein could dis-tinguish sera from tuberculosis patients with anti-PPD antibody and those from tuberculin positive contacts.Con-clusions:We get recombinant MPT64protein highly expressed in E.coli.This recombinant MPT64showes specific immunogenicity and can be a new serodiagnostic reagent." @default.
- W2380966032 created "2016-06-24" @default.
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- W2380966032 date "2004-01-01" @default.
- W2380966032 modified "2023-09-25" @default.
- W2380966032 title "Expression of mycobacterium tuberculosis MPT64in E.coli,purification and application to sera diagnose" @default.
- W2380966032 hasPublicationYear "2004" @default.
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