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- W2380971491 abstract "Objective To develop and evaluate a rapid DNA extraction method using Whatman FTA cards,in molecular identification of fungi. Methods DNA was extracted from 45 isolates encompassing 25 species and 6 clinical samples by whatman FTA cards. Then PCR amplification and sequencing of the internal transcribed spacer region 1 (ITS1) were conducted. Serial diluted suspension of conidia was prepared to determine the detection limit and security of this method. Results A clear DNA amplified fragment was obtained and successfully sequenced in all 45 isolates from clinical samples. The detection limit was approximately 103 cell/ mL cembined with touchdown PCR. Suspension with less than 104 cell/mL conidia could be thoroughly inactivated by FTA cards. Conclusions Whatman FTA technology thus represents an ultra-rapid method of fungal genomic DNA preparation for molecular identification from both cultures and clinical samples,and also potentially represents a powerful fungal DNA archiving and storage system." @default.
- W2380971491 created "2016-06-24" @default.
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- W2380971491 date "2010-01-01" @default.
- W2380971491 modified "2023-10-03" @default.
- W2380971491 title "A rapid DNA extraction method for molecular identification of pathogenic fungi" @default.
- W2380971491 hasPublicationYear "2010" @default.
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