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- W2380973389 abstract "Objective To study the effect of mechanical ventilation on the expression of MUC5AC and investigation for mechanism.Methods Rabbits were divide into control group and test groups including one,three,six,twelve,twenty-four hour mechanical ventilation and 2,5,10 cm H2O PEEP mechanical ventilation pressure.Contents of TNF-α,IL-8,MUC5AC mRNA and protein in bronchoalveolar lavage fluid were determined by RT-PCR and ELISA methods,respectively.Neutrophils in bronchoalveolar lavage fluid were counted.Neutrophil elastase activity was measured by assays of substrate.Results Mechanical ventilation increased expression of MUC5AC in bronchiorum endothelial cells(P0.05).After mechanical ventilation for three hours,the expression of TNF-α reached the peak(P0.05).The expression of IL-8 reached the peak after six hours(P0.05).Neutrophil count and neutrophil elastase activity reached the peak after twelve hours(P0.05).With the increase of PEEP,the mRNA and protein of TNF-α,IL-8,MUC5AC,the number of PMN and neutrophil elastase activity was also increased(P0.05).Conclusion Mechanical ventilation may increase the expression of MUC5AC in bronchiorum endothelial cells.This maybe is concerned with inflammation." @default.
- W2380973389 created "2016-06-24" @default.
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- W2380973389 date "2011-01-01" @default.
- W2380973389 modified "2023-09-27" @default.
- W2380973389 title "Effects of mechanical ventilation by breathing machine on the expression of MUC5AC in rabbits" @default.
- W2380973389 hasPublicationYear "2011" @default.
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