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- W2380976499 abstract "Objective To find rapid and reliable molecular methods to identify medically important Fusarium spp..Methods The Fusarium spp.tested were cultured on PDA.Their ITS of rDNA were amplified using PCR and their PCR products were sequenced.BLAST and sequences analysis were performed through the nucleic acid bank of the GenBank.Endonuclease including Dra II and Cfr13 I were selected to perform RFLP analysis.A pair of species-specific primers-Sol1 and Sol2 of F.solani were designed and their specificity were tested.Results F.solani were the most predominant species based on morphological characters.The sequences of ITS analysis results were coincident with their morphologies except two F.moniliforme.The RFLP patterns of F.solani,F.moniliforme and F.proliferatum digested by Dra II and Cfr13 I were different from each other.The species-specific primers can only amplify rDNA of F.solani.Conclusions PCR-RFLP and sequencing of rDNA ITS can identify some Fusarium spp..Appropriate species-specific primers can be used to identify Fusarium solani rapidly." @default.
- W2380976499 created "2016-06-24" @default.
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- W2380976499 date "2008-01-01" @default.
- W2380976499 modified "2023-09-25" @default.
- W2380976499 title "Identification of clinically important Fusarium spp." @default.
- W2380976499 hasPublicationYear "2008" @default.
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