Matches in SemOpenAlex for { <https://semopenalex.org/work/W2380981919> ?p ?o ?g. }
Showing items 1 to 57 of
57
with 100 items per page.
- W2380981919 abstract "Objective:To investigate the expression, cellular and subcellular localization of the novel mouse Ty21a immunization_associated gene (TIG_310) and to find out its variation during the Ty21a immunization. Methods:The coding region of TIG_310 was gained by PCR and was then inserted into pQE30 vector. The expression of TIG_310 in E.coli M15 was induced by IPTG at 37℃and was then analyzed by SDS_PAGE. The cellular localization information was obtained by in situ hybridization. To obtain the subcellular localization of TIG_310, the gene was subcloned into the pEGFP_N1 vector and then transfected into L cells. The expression of fusion protein was analyzed by confocal microscopy. The information about TIG_310 expression during the Ty21a immunization was obtained by RT_PCR. Results and Conclusions:The TIG_310 gene was successfully expressed in E.coli M15. The expressed protein existed in the inclusion body. In situ analysis showed that the TIG_310 gene was mainly in the epithelial cells of the gastrointestinal tract. The protein coded by TIG_310 gene distributed through out the cells. The expression of TIG_310 was elevated after Ty21a immunization." @default.
- W2380981919 created "2016-06-24" @default.
- W2380981919 creator A5024789842 @default.
- W2380981919 date "2003-01-01" @default.
- W2380981919 modified "2023-09-22" @default.
- W2380981919 title "Expression,cellular and subcellular localization of the novel gene TIG-310 and its variation in the process of Ty21a immunization" @default.
- W2380981919 hasPublicationYear "2003" @default.
- W2380981919 type Work @default.
- W2380981919 sameAs 2380981919 @default.
- W2380981919 citedByCount "0" @default.
- W2380981919 crossrefType "journal-article" @default.
- W2380981919 hasAuthorship W2380981919A5024789842 @default.
- W2380981919 hasConcept C104317684 @default.
- W2380981919 hasConcept C123894998 @default.
- W2380981919 hasConcept C150194340 @default.
- W2380981919 hasConcept C153911025 @default.
- W2380981919 hasConcept C2776879804 @default.
- W2380981919 hasConcept C40767141 @default.
- W2380981919 hasConcept C54009773 @default.
- W2380981919 hasConcept C54355233 @default.
- W2380981919 hasConcept C86803240 @default.
- W2380981919 hasConceptScore W2380981919C104317684 @default.
- W2380981919 hasConceptScore W2380981919C123894998 @default.
- W2380981919 hasConceptScore W2380981919C150194340 @default.
- W2380981919 hasConceptScore W2380981919C153911025 @default.
- W2380981919 hasConceptScore W2380981919C2776879804 @default.
- W2380981919 hasConceptScore W2380981919C40767141 @default.
- W2380981919 hasConceptScore W2380981919C54009773 @default.
- W2380981919 hasConceptScore W2380981919C54355233 @default.
- W2380981919 hasConceptScore W2380981919C86803240 @default.
- W2380981919 hasLocation W23809819191 @default.
- W2380981919 hasOpenAccess W2380981919 @default.
- W2380981919 hasPrimaryLocation W23809819191 @default.
- W2380981919 hasRelatedWork W2248025878 @default.
- W2380981919 hasRelatedWork W2348598734 @default.
- W2380981919 hasRelatedWork W2349810131 @default.
- W2380981919 hasRelatedWork W2354789566 @default.
- W2380981919 hasRelatedWork W2355374881 @default.
- W2380981919 hasRelatedWork W2356803921 @default.
- W2380981919 hasRelatedWork W2359362944 @default.
- W2380981919 hasRelatedWork W2361285893 @default.
- W2380981919 hasRelatedWork W2366367467 @default.
- W2380981919 hasRelatedWork W2371864295 @default.
- W2380981919 hasRelatedWork W2375301618 @default.
- W2380981919 hasRelatedWork W2376565780 @default.
- W2380981919 hasRelatedWork W2376851738 @default.
- W2380981919 hasRelatedWork W2381594337 @default.
- W2380981919 hasRelatedWork W2381981384 @default.
- W2380981919 hasRelatedWork W2383744146 @default.
- W2380981919 hasRelatedWork W2390792353 @default.
- W2380981919 hasRelatedWork W2392354016 @default.
- W2380981919 hasRelatedWork W3036301936 @default.
- W2380981919 hasRelatedWork W33738171 @default.
- W2380981919 isParatext "false" @default.
- W2380981919 isRetracted "false" @default.
- W2380981919 magId "2380981919" @default.
- W2380981919 workType "article" @default.