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- W2380987420 abstract "Objective To study morphology and function of dendritic cells in injection site induced by DNA vaccine derived from Japanese encephalitis virus. Methods BALB/c mice were vaccinated with DNA vaccine(pJME) of Japanese Encephalitis virus prM、E encoding protein via intramuscular injection. The morphology of local infiltration cells in injection sites was observed through HE staining,and infiltration cells were further studied with the dendritic cell marker CD11b and CD11c by immunohistochemical methods and with DC mature markers CD80 and CD86 by flow cytometry technique. Results The grading of muscle infiltrates(3.0+) and the grading of CD11b+ and CD11c+ cell percentage (2.0+,2.0+, respectively) produced by pJME via intramuscular injection were significantly higher than those by inactivated vaccine and vector(P 0.05),and there was no significant difference in the expressions of CD80 and CD86 on dendritic cells in injection site between pJME (0.16±0.20)%,inactivated vaccine (0.15±0.25)% and vector (0.12±0.30)%(P 0.05). Conclusion pJME can induce many kinds of cell infiltration in injection sites,including immature myeloid dendritic cells." @default.
- W2380987420 created "2016-06-24" @default.
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- W2380987420 date "2008-01-01" @default.
- W2380987420 modified "2023-09-24" @default.
- W2380987420 title "Local Infiltration of Dendritic Cells in Injection Site of BALB/c Mice Induced by DNA Vaccine Derived from Japanese Encephalitis Virus" @default.
- W2380987420 hasPublicationYear "2008" @default.
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