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- W2381004022 abstract "Objective To purify a peptide from Agkistrodon Acutus snake venom and analyse its characteristics and biological activities.Methods To extract snake venom through 75 gel filtration,ultrafiltration and DEAE-Sepharose CL-6B ionexchange.The purified product was identified by HPLC.The molecular weight was determined by SDS-polyacrylamid gel electrphoresis.The isoelectric point was estimated by the isoelectric focusing electrophoresis.Platelet aggragation was measured by nephelometry.The antiangiogenesis effect in vivo of peptide on CAM assay was observed.Results The molecular weight of peptide purified from Agkistrodon Acutus snake venom was 7 862D.Its isoelectric point was pH4.29.This peptide could dose-dependently inhibited the platelet aggregation induced by ADP,collagen and thrombin.It inhibit the spontaneous angiogenesis of CAM.Conclusion The method has been proved to be successful for the purification of peptide that inhibits platelet aggregation and angiogenesis" @default.
- W2381004022 created "2016-06-24" @default.
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- W2381004022 date "2007-01-01" @default.
- W2381004022 modified "2023-09-23" @default.
- W2381004022 title "The Study of Peptide in Agkistrod on Acutus Snake Venom" @default.
- W2381004022 hasPublicationYear "2007" @default.
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