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- W2381033326 abstract "Lymphoid tissues from thymus and bursa Fabricus of chicken were sheared and mixed with PBS buffer.T lymphocytes and B lymphocytes isolated from the lymphoid tissues were purified by density gradient centrifugation and nylon wool chromatography and stained with mouse-anti-chicken's CD4-FITC,CD8-RPE and Bu-1-FITC monoclonal antibodies,respectively.The purity and activity of the T and B lymphocytes were examined using flow cytometry and trypan blue exclusion assay,respectively.The results showed that the purity of T and B cells purified with the above mentioned method was more than 90% and their activities were similar to that of the cells isolated with flow cytometry." @default.
- W2381033326 created "2016-06-24" @default.
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- W2381033326 date "2007-01-01" @default.
- W2381033326 modified "2023-09-23" @default.
- W2381033326 title "Establishment of simple method for purification of T and B lymphocytes of chicken" @default.
- W2381033326 hasPublicationYear "2007" @default.
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