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- W2381036664 abstract "sHSPs is a structurally divergent group,inducing to composition when cell and biosystem are stressed by variety of conditions.This kind of protein has been studied less than others currently.The authors analyze the bioinformation of a cDNA sequence selected from silkworm pupa cDNA library(constructed by our laboratory) and find that this protein belongs to HSP family.The authors named it BmHSP22.6.The ORF of BmHSP22.6 gene is amplified by PCR and inserted into the pET28a(+) expression vector.Following transformation of E.coli BL21(DE3) cells with the pET28a(+)-BmHSP 22.6 recombinates plasmid. IPTG with final concentration of 1 mM is added to the culture medium to induce the expression of recombinant protein.The interest protein is harvested by eluted with Nickel-affinity chromatography column." @default.
- W2381036664 created "2016-06-24" @default.
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- W2381036664 date "2010-01-01" @default.
- W2381036664 modified "2023-09-28" @default.
- W2381036664 title "Analysis of Bioinformatics and Prokaryotic Expression of Bombyx mori HSP22.6 Gene" @default.
- W2381036664 hasPublicationYear "2010" @default.
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