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- W2381042080 abstract "Objective To establish a method for rapid detection of clinical suspect fungal corneal ulcer by polymerase chain reaction(PCR).Methods A pair of oligonucleotide sequences,which was bases on the conserved region of 18srRNA shared by medically important conditioned fungal,was used as the general primers to amplify the DNAs from clinical suspect fungal corneal ulcer in a PCR assay,and the result was contrasted with culture.Results A 400bp specific DNA product was successfully amplified.The positive rate of fungi culture among 36 clinical specimens was 58.3%,while that of PCR amplification was 86.1%.In addition,the accuracy of PCR method in this study was 72.2%,the sensitivity was 100%,and the specialty was 33.3%.Conclusion PCR with the general primers is suitable for rapid detection of fungal corneal ulcer because of quickness and high positive rate." @default.
- W2381042080 created "2016-06-24" @default.
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- W2381042080 date "2009-01-01" @default.
- W2381042080 modified "2023-09-24" @default.
- W2381042080 title "Applied polymerase chain reaction detecting fungal corneal ulcer" @default.
- W2381042080 hasPublicationYear "2009" @default.
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