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- W2381058438 abstract "In order to optimize the fermentation conditions of Bacillus sp.BⅡ-5,a thermophilic strain from Indonesia hot spring,effects of medium composition and culture condition on agarase production by Bacillus sp.SR-B3 was investigated.Based on the former experiments,yeast extract,peptone,agar,calcium chloride,initial pH,the inoculum size and NaCl were selected as the main factors that influence the agarase activity.Peptone,agar and calcium chloride were elected as the three more important factors by using Plackett-Burman design.The experimental design of steepest ascent was used to approach the optimal region of the three significant factors.Box-Behnken design was adopted to analysis the mutual interactions between the variables and obtain the optimal values that could bring the maximum agarase activity.The optimal medium composition for agarase production were: Yeast extract 0.4000%,peptone 0.6650%,agar 1.1600‰,calcium chloride,7.0800mmol/L,initial pH value 7.5,1% of the inoculum size and NaCl added 6‰.Mathematical and statistical methods are used to optimize the fermentation condition of Bacillus sp.BⅡ-5 producing agarase and establish mathematical models,this laid a theoretical basis for agarase industrial applications of Bacillus sp.BⅡ-5." @default.
- W2381058438 created "2016-06-24" @default.
- W2381058438 creator A5052477693 @default.
- W2381058438 date "2013-01-01" @default.
- W2381058438 modified "2023-09-22" @default.
- W2381058438 title "Optimization of medium composition and culture conditions for agarase production by Bacillus sp. BII-5" @default.
- W2381058438 hasPublicationYear "2013" @default.
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