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- W2381061021 abstract "In this study,a small RNA library mixed equally sRNA of needle leaves,stem and root tissues was constructed for 2-year-old seedling of Japanese larch(Larix kaempferi).Two hundred and eighty three recombinants from library were partially selected randomly and sequenced.These sequenced sequences were searched and compared in Rfam,Repbase,miRBase and PMRD databases by BLASTN to remove rRNA,tRNA,snRNA snoRNa and other non-candidate small RNA.The results indicated that 28 miRNAs were acquired and these sequences were same(miR159c,miR160a,miR162a,miR164b,miR165a,miR166a,miR166a*,miR166b*,miR169a,miR169b,miR171a,miR171b,miR172a,miR319b,and miR396a) as or highly homologous(miR156a,miR159a,miR159b,miR164a,miR166b,miR168a,miR169b*,miR319a,miR396b,miR408,miR482a,miR2111,and miR3701) to other known plant miRNAs,which belonging to 17 miRNA families.Additional 4 sequences could be matched to stem-arm sequences of stem-loop structure M-folded with our lab's transcriptome data(contigs/singlets).The 4 sequences could be characterized novel miRNAs of Japanese larch.Quantitative real-time polymerase chain reaction(qPT-PCR) analysis demonstrated that 20 miRNAs were expressed in larch.The putative target genes of these miRNAs were predicted using psRNATarget online service.There were 69 targets obtained by the prediction for 24 miRNAs among 28 conserved and 4 novel larch miRNAs,which are highly enriched in transcription factor(TF),signal transduction protein,stress-induced-associate protein and other uncharacterized protein." @default.
- W2381061021 created "2016-06-24" @default.
- W2381061021 creator A5052883436 @default.
- W2381061021 date "2012-01-01" @default.
- W2381061021 modified "2023-09-25" @default.
- W2381061021 title "Construction of Small RNA Library and Characterization of MiRNAs in Larix kaempferi" @default.
- W2381061021 hasPublicationYear "2012" @default.
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