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- W2381096005 abstract "【Objective】This study aims to screen Fusarium head blight(FHB) resistance plants in Hpa110-42 transgenic wheat plants and provide materials for FHB resistance breeding of wheat.【Method】T2 transgenic wheat plants were identified by PCR,Southern blot and RT-PCR,assuring whether the foreign Hpa110-42gene was integrated and expressed in transgenic wheat plants.The resistance to FHB of transgenic wheat plants was evaluated by a dripping method to single flower,and its resistance physiology was studied.【Result】PCR,Southern blotting and RT-PCR analyses have confirmed successful integration of the foreign Hpa110-42gene into the genome of the Yangmai 158 with 1 to 3 copies and stable inheritance and expression.Disease bioassays of transgenic plants revealed that the average percentage of diseased spikelets of transgenic lines T2-17,T2-15,T2-68,T2-44 and T2-36 were highly significantly lower than YM158.Compared with Sumai 3,the average percentage of diseased spikelets of all transgenic lines was highly significantly higher than Sumai 3 excepting line T2-17.In the transgenic lines,the average percentage of diseased spikelets of line T2-17 was significantly lower than other lines,in which T2-36,T2-11 and T2-20 have reached highly significant difference level.It is showed by physiological analysis that the activities of PAL,chitinase and β-1,3-glucanase in the high resistance transgenic plants increased faster than Yangmai 158 after inoculation.The soluble protein content in all plants had a downward trend,while its content in high resistance plants is consistently higher than others.Moreover,β-1,3-glucanase activity and soluble protein content were negatively correlated with resistance grade of FHB.【Conclusion】The foreign Hpa110-42 gene was stably inherited,normally expressed and positively participated in the regulation of FHB resistance,and Hpa110-42 transgenic wheat plants with resistance to FHB were obtained." @default.
- W2381096005 created "2016-06-24" @default.
- W2381096005 creator A5058343469 @default.
- W2381096005 date "2013-01-01" @default.
- W2381096005 modified "2023-09-26" @default.
- W2381096005 title "The Molecular Identification of Transgenic Hpa1_(10-42) Wheat and Resistance Evaluation on Fusarium Head Blight" @default.
- W2381096005 hasPublicationYear "2013" @default.
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