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- W2381097232 abstract "Objective To set up a real-time PCR with Taq enzyme probe for the detection of hepatitis B virus covalently closed circular DNA(cccDNA).Methods Two primers were designed according to structure difference between rcDNA and cccDNA for PCR and TaqMan probe was applied for analysis.Subsequently,PCR products were sequencing for confirmation.Eighty nine serum samples in line with the hepatitis B diagnostic criteria and three samples of healthy people were collected.The samples were quantified for HBV cccDNA and DNA.Results Amplified products were confirmed to be the target segment by sequencing.The lower detection limit was 500copies/ml.The HBV cccDNA positive rates were 55.0%,67.8% and 30.0% in all CHB patients,HBeAg positive patients and HBeAb positive patients,respeitively.The cccDNA positive rate in HBeAg positive patients was significantly higher than that in HBeAb positive patients(P0.05).No HBV cccDNA was detected in healthy people.According to ROC curve evaluation,the value of the HBV cccDNA for antiviral therapy was 0.927.Conclusion A successful real-time PCR detection method with the TaqMan probe to detect the serum of HBV cccDNA is set up with fast,high specificity and high reference value.Quantifying of HBV cccNDA by this method can provide important reference index of predicating the effect of clinical anti-viral treatment and guide clinical medication." @default.
- W2381097232 created "2016-06-24" @default.
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- W2381097232 date "2010-01-01" @default.
- W2381097232 modified "2023-09-23" @default.
- W2381097232 title "Quantitative detection and application of HBV cccDNA" @default.
- W2381097232 hasPublicationYear "2010" @default.
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