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- W2381327057 abstract "OBJECTIVE To understand the effect of dual real-time PCR in detecting Samonella and Shigella. METHODS The samples were collected from rectal swabs of employees then samples of Samonella and Shigella were detected by real-time PCR. According to the national standards, the probable positive samples were cultured by traditional culture method. RESULTS The dual real-time PCR assay was used to detect 39 974 samples of employees. 41 and 31 Salmonella were identified by real-time PCR and traditional culture method respectively and the positive rates were 1.22‰ and 0.78‰ respectively. The sensitivity and specificity of real-time PCR assay for detecting Salmonella were 100% and 99.95%. 10 and 3 Shigella were identified by real-time PCR and traditional culture method respectively and the positive rates were 0.25‰ and 0.08‰ respectively. The sensitivity and specificity of by real-time PCR assay for detecting Shigella were 100% and 99.88%. The overall test could be finished from 2 hours to one day starting from sample preparation. CONCLUSION The real-time PCR assay is a rapid, sensitive and specific method for detecting Samonella and Shigella which establishes an important foundation for rapid diagnosis of intestinal infectious diseases." @default.
- W2381327057 created "2016-06-24" @default.
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- W2381327057 date "2013-01-01" @default.
- W2381327057 modified "2023-09-23" @default.
- W2381327057 title "Analysis of rapid detection results of Samonella and Shigella by real-time PCR" @default.
- W2381327057 hasPublicationYear "2013" @default.
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