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- W2381340507 abstract "By embryo in vitro culture method,change of some physiological-biochemical indexes and correlation among these indexes during germination process of embryos in vitro of Cyclocarya paliurus(Batal.) Iljinsk.were analyzed under the condition of adding 81.33 mg·L-1(hemi-inhibitory concentration,IC50) vanillic acid(VA) in medium.The results show that with prolonging of culture time,contents of crude fat and protein in embryos in vitro of treatment group(adding 81.33 mg·L-1 VA in medium) and the control group all appear the trend of decreasing gradually,while contents of starch and soluble sugar all appear the trend of decreasing at first and then increasing,activities of isocitrate lyase(ICL),acid phosphatase(AP),alkaline phosphatase(ALP) and peroxidase(POD),combined activity of glucose-6-phosphate dehydrogenase(G-6-PDH) and 6-phosphogluconate dehydrogenase(6-PGDH) all are higher than those at start culture stage,and activities of ICL,POD,AP and ALP all increase continuously.But contents of crude fat and protein in embryos in vitro of treatment group are both higher than those of the control group and their change ranges are relatively smaller,starch content is higher at prophase but lower at anaphase than that of the control,soluble sugar content is generally higher than that of the control,enhancement extent of activities of ICL,AP and ALP,combined activity of G-6-PDH and 6-PGDH are generally lower than those of the control,POD activity is significantly higher than that of the control at anaphase.The correlation analysis result shows that there are significant or extremely significant negative correlations among contents of crude fat and protein with activities of above-mentioned enzymes.It is considered that VA has an inhibition to activations of enzymes during germination process of embryos in vitro of C.paliurus and leads to hinder from the transformation of main storage substances,so as to inhibit germination of embryos." @default.
- W2381340507 created "2016-06-24" @default.
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- W2381340507 date "2012-01-01" @default.
- W2381340507 modified "2023-09-24" @default.
- W2381340507 title "Effect of vanillic acid on physiological-biochemical indexes of embryos in vitro of Cyclocarya paliurus during germination process and correlation analysis of these indexes" @default.
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