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- W2381340714 abstract "Objective To establish a real-time quantitative RT-PCR method for the detection of expression of TNF-α mRNA.Methods The vector containing TNF-α gene as standard template was constructed with T-A cloning technique.The fluorogenic probe(i.e,Taqman probe)was used to establish a real-time RT-PCR.TNF-α mRNA expression level in human peripheral blood mononuclear cells(PBMC) for tumor patients and normal persons was determined with real-time quantitative RT-PCR,also with semi-quantitative RT-PCR.Results The expression of TNF-α mRNA in PBMC for tumor patients was more high than that of normal persons;The semi-quantitative RT-PCR results also demonstrated that TNF-α level varied as detected with real-time fluorogenic quantitative RT-PCR,but less sensitive and accurate.Conclusion Detection of TNF-α mRNA expression with the fluorogenic real-time quantitative RT-PCR is more accurate and sensitive than semi-quantitive RT-PCR." @default.
- W2381340714 created "2016-06-24" @default.
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- W2381340714 date "2006-01-01" @default.
- W2381340714 modified "2023-09-23" @default.
- W2381340714 title "Real-time Quantitative RT-PCR for the Detection of TNF-α mRNA Expression" @default.
- W2381340714 hasPublicationYear "2006" @default.
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