Matches in SemOpenAlex for { <https://semopenalex.org/work/W2381385267> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2381385267 abstract "Aim: To construct the prokaryotic expression vector for HLA-A*1101 heavy chain ectodomain fused with a BirA substrate peptide(BSP) at its carboxyl terminus and express the fusion protein in Escherichia coli(E.coli).Methods: The cDNA of the heavy chain gene of HLAA*1101 was cloned by RT-PCR from HLA-A2-donors and verified by DNA sequencing. An expression vector,which encoded the ectodomain of HLA-A*1101 fused with BSP at the carboxyl terminus,was constructed by PCR with the cloned cDNA as a template.The recombinant protein was expressed in E.coli and identified with Western blotting.Results: The cDNA of HLA-A*1101 heavy-chain gene was cloned from peripheral blood mononuclear cells of HLA-A2-donors.The expression vector for the fusion protein of the ectodomain(residues 1-276) of HLA-A*1101 fused with BSP at the carboxyl terminus was constructed and the recombinant plasmid was verified by DNA sequencing.The fusion protein was over expressed in E.coli BL21(DE3),which accounted for 20% of total bacterial proteins.The relative molecular weight of this fusion protein was 35 000,which is consistent with the theoretical value.Western blotting showed that all of the fusion protein existed in the inclusion bodies,without any products in the supernatant.Conclusion: The prokaryotic expression vector for HLA-A11BSP has been constructed and the fusion protein is expressed in high yield in the form of inclusion bodies in E.coli." @default.
- W2381385267 created "2016-06-24" @default.
- W2381385267 creator A5038668046 @default.
- W2381385267 date "2006-01-01" @default.
- W2381385267 modified "2023-09-25" @default.
- W2381385267 title "Construction and expression of the prokaryotic expression vector for high frequency allele HLA-A* 1101 heavy-chain ectodomain fused with BSP" @default.
- W2381385267 hasPublicationYear "2006" @default.
- W2381385267 type Work @default.
- W2381385267 sameAs 2381385267 @default.
- W2381385267 citedByCount "0" @default.
- W2381385267 crossrefType "journal-article" @default.
- W2381385267 hasAuthorship W2381385267A5038668046 @default.
- W2381385267 hasConcept C1009742 @default.
- W2381385267 hasConcept C104317684 @default.
- W2381385267 hasConcept C123894998 @default.
- W2381385267 hasConcept C153911025 @default.
- W2381385267 hasConcept C170493617 @default.
- W2381385267 hasConcept C187882448 @default.
- W2381385267 hasConcept C33161422 @default.
- W2381385267 hasConcept C40767141 @default.
- W2381385267 hasConcept C547475151 @default.
- W2381385267 hasConcept C55493867 @default.
- W2381385267 hasConcept C85265743 @default.
- W2381385267 hasConcept C86803240 @default.
- W2381385267 hasConceptScore W2381385267C1009742 @default.
- W2381385267 hasConceptScore W2381385267C104317684 @default.
- W2381385267 hasConceptScore W2381385267C123894998 @default.
- W2381385267 hasConceptScore W2381385267C153911025 @default.
- W2381385267 hasConceptScore W2381385267C170493617 @default.
- W2381385267 hasConceptScore W2381385267C187882448 @default.
- W2381385267 hasConceptScore W2381385267C33161422 @default.
- W2381385267 hasConceptScore W2381385267C40767141 @default.
- W2381385267 hasConceptScore W2381385267C547475151 @default.
- W2381385267 hasConceptScore W2381385267C55493867 @default.
- W2381385267 hasConceptScore W2381385267C85265743 @default.
- W2381385267 hasConceptScore W2381385267C86803240 @default.
- W2381385267 hasLocation W23813852671 @default.
- W2381385267 hasOpenAccess W2381385267 @default.
- W2381385267 hasPrimaryLocation W23813852671 @default.
- W2381385267 hasRelatedWork W2347299188 @default.
- W2381385267 hasRelatedWork W2349480482 @default.
- W2381385267 hasRelatedWork W2350146130 @default.
- W2381385267 hasRelatedWork W2354307296 @default.
- W2381385267 hasRelatedWork W2358393024 @default.
- W2381385267 hasRelatedWork W2366287568 @default.
- W2381385267 hasRelatedWork W2368186429 @default.
- W2381385267 hasRelatedWork W2368475441 @default.
- W2381385267 hasRelatedWork W2368672550 @default.
- W2381385267 hasRelatedWork W2370397273 @default.
- W2381385267 hasRelatedWork W2372927515 @default.
- W2381385267 hasRelatedWork W2374007278 @default.
- W2381385267 hasRelatedWork W2375907603 @default.
- W2381385267 hasRelatedWork W2383175631 @default.
- W2381385267 hasRelatedWork W2384703308 @default.
- W2381385267 hasRelatedWork W2386842231 @default.
- W2381385267 hasRelatedWork W2387360189 @default.
- W2381385267 hasRelatedWork W2391157018 @default.
- W2381385267 hasRelatedWork W2392506252 @default.
- W2381385267 hasRelatedWork W3146252746 @default.
- W2381385267 isParatext "false" @default.
- W2381385267 isRetracted "false" @default.
- W2381385267 magId "2381385267" @default.
- W2381385267 workType "article" @default.